Onset and Heterogeneity of Responsiveness to FSH in Mouse Preantral Follicles in Culture

P-107: Vitrification and In Vitro Culture of Mouse Preantral Follicles in Presence of Growth Factors

Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...

Effect of Fibroblast Co-culture on In Vitro Maturation and Fertilization of Mouse Preantral Follicles

P-83: Development of Mouse Preantral Follicles in Fibrin-Alginate Matrix during In Vitro Culture

Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged...

P-116: Maturation Genes Expression during In Vitro Culture of Vitrified Mouse Preantral Follicles

Background: Evaluation of maturation genes expression and their pattern during in vitro culture of vitrified preantral follicles is an essential and could promote either vitrification procedure or culture condition. Materials and Methods: Preantral follicles (with 100- 130 μm diameter) isolated mechanically from 12-14 days old NMRI mice ovaries and divided into vitrification and control groups....

In vitro culture of goat preantral follicles

Background: The majority of thousands of oocytes in the ovaries are small, non-growing and reside in preantral follicles (PFs). The development of a culture system for preantral follicle may be very useful for understanding the complex mechanism in folliculogenesis at early stages of development as wells as could offer a significant way for the propagation of livestock, including goats. This pa...